Exo User Manual Chlorophyll Calibration Rhodamine

Calibration menus quickly guide you through the calibration process and provide SmartQC verification of successful calibration and worksheets. EXO Sondes: What's in the Box When it Arrives? In this unboxing video, Tim Finegan, YSI, shows you exactly what ships with every order of an EXO water quality monitoring sonde. Experienced user with the steps. Required to make your YSI 6-Series. Product operational. For full instructions, please refer to. The 6-Series User Manual. Optical probes (6025 chlorophyll, 6130. Rhodamine, 6131 phycocyanin blue-green. Algae, 6132 phycoerythrin blue-green algae, 6136 turbidity, and 6150 ROX DO): Insert. EXO chlorophyll readings show excellent linearity on serial dilution of a surrogate solution of Rhodamine WT (R 2 0.9999) and this should ensure relative accuracy of field chlorophyll readings, i.e., a chlorophyll reading of 100 units will represent twice the algal content of water with a chlorophyll reading of 50 units. Self-monitoring system reports the status of the instrument, flags the data, and shows the user where the problem is with assistance on how to solve the issue; User-scheduled calibration and maintenance intervals indicate when they are due; Guided and semi-automated calibration routines lead the user through the calibration process. The EXO handheld is a portable weatherproof interface for the EXO sondes that also comes with a number of unique features. These features include GPS, temperature-compensated barometer, wet-mate wireless connector, Bluetooth communications, and 2 GB of storage. Besides the handheld, the EXO sonde has multiple data outputs.

*Specifications indicate typical performance and are subject to change. All sensors have a depth rating to 250 m (820 ft), except shallow and medium depth sensors, ammonium, chloride, and nitrate. Accuracy specification is attained immediately following calibration under controlled and stable environmental conditions. Performance in the natural environment may vary from quoted specification.
w.i.g. = whichever is greater
EXO sensors are not compatible with YSI 6-Series sondes, sensors, or handheld.
1Outputs of specific conductance (conductivity corrected to 25˚C) and total dissolved solids are also provided. See Calculated Parameters and footnote 11.
2 Temperature accuracy traceable to NIST standards
3Accuracy specifications apply to conductivity levels of 0 to 100,000 μS/cm.
4 Relative to calibration gases
5 When transferred from air-saturated water to stirred deaerated water
6 When transferred from water-saturated air to Zobell solution
7Within the environmental pH range of pH 4 to pH 10.
8On transfer from water-saturated air to rapidly stirred air-saturated water at a specific conductance of 800 μS/cm at 20˚C; T63<5 seconds on transfer from water-saturated air to slowly-stirred air-saturated water.
9 Performance based on 3-point calibration done with YSI AMCO-AEPA standards of 0, 124, and 1010 FNU. The same type of standard must be used for all calibration points.
10 Values are automatically calculated from conductivity according to algorithms found in Standard Methods for the Examination of Water and Wastewater (Ed. 1989).

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Unpacking and Inspection

Inspect the outside of the shipping

container(s) for damage. If you see any

damage, contact your shipping carrier

immediately.

Remove the equipment from the shipping

box. Some parts are loose in the box, so check

the packing material carefully. Check off all

items on the packing list and inspect them for

damage.

Items Needed

Sonde

• Probes

• Calibration cup

• Probe guard

• Batteries (if sonde has battery compartment)

• DI water

• Flathead screwdriver

• Calibration standards

• Communication or field cable

If using 6562 DO probe:

• 6570 maintenance kit

• 5775 membrane kit

Exo User Manual Chlorophyll Calibration Rhodamine Guide

Exo user manual chlorophyll calibration rhodamine 2

• Razor blade

6-Series Sonde

Quick Start Guide

Preparing Sonde for Use

To prepare the sonde for operation, you

will need to:

• Install a new membrane on the YSI 6562

DO Probe, if you are using this item.

• Install probes (sensors) into the connectors

on the sonde bulkhead.

• Provide power for the sonde through

batteries or line power.

• Connect a field cable or PC communication

cable.

It is important to calibrate all

sensors prior to using the sonde for field

measurements. Refer to Section 2.6.2 of the

6-Series User Manual.

Installing Membrane on

6562 DO Probe

(Skip to next section if you are not using

this probe)

The 6562 Rapid Pulse

Dissolved Oxygen

probe is shipped with a protective dry

membrane on the sensor tip. Remove the

O-ring and membrane. Handle the probe

with care. It is important not to scratch or

contaminate the sensor tip.

The DO sensor on the 600R/QS cannot

be removed.

Open the 6562 DO Probe Kit and follow these

steps:

1. Prepare the electrolyte solution as specified

on the bottle.

2. Put/keep the protective cap on the

connector end of the probe to protect it from

contamination.

3. Hold the probe in a vertical position and

apply a few drops of KCl solution to tip. The

fluid should completely fill the small moat

around the electrodes—and create a large

meniscus on the probe face. Be sure no air

bubbles are stuck to the face of the sensor. If

necessary, shake off electrolyte and start over.

4. Secure a membrane between your left

Exo User Manual Chlorophyll Calibration Rhodamine 1

thumb and the probe body. Touch membrane

only at the ends.

5. With the thumb and forefinger of

your right hand, grasp the free end of the

membrane and gently stretch it up, over,

and down the other side of the sensor. The

membrane should be stretched below the

ridge where the O-ring seats so a good seal is

achieved.

6. Secure the end of the membrane under the

forefinger of your left hand.

7. Roll the O-ring over the end of the probe,

being careful not to touch the membrane

surface with your fingers.

8. If bubbles or wrinkles are present, remove

the membrane and repeat steps 3-7.

9. Trim excess membrane with a razor blade.

Rinse off any excess KCl solution, but be

careful not to get any water in the connector.

Refer to Section 2.3 of the 6-Series User

Manual for complete instructions and photos.

Installing Probes

Before installing any probe into the

sonde bulkhead, be sure that the probe port

is free of moisture. Use a Kimwipe

or lint-

free cloth to dry.

1. Remove the calibration cup from your

sonde, if necessary.

2. Using the long end of the probe

installation tool supplied in the 6570

Maintenance Kit, remove the port plug from

the port.

3. Some probes can only be installed into

specific ports. Refer to Section 2.3 of the 6-

Series User Manual for port designations.

4. Apply a thin coat of lubricant (Krytox

)

to the O-rings on the connector side of the

probe.

Make sure there are NO contaminants

between the O-ring and the probe.

Contaminants will cause leaks when the

sonde is deployed.

When installing probes, be
careful not to cross-thread the
probe nuts. If you meet resistance
while finger-tightening, STOP,
back out, and begin again.

This document will assist an

experienced user with the steps

required to make your YSI 6-Series

product operational.

For full instructions, please refer to

the 6-Series User Manual.

5. Optical probes (6025 chlorophyll, 6130

Rhodamine, 6131 phycocyanin blue-green

algae, 6132 phycoerythrin blue-green algae,

6136 turbidity, and 6150 ROX DO): Insert

the probe in the appropriate optical port,

seating the pins of the two connectors. Hand-

tighten the probe nut to the bulkhead. Finish

tightening the probe using the short end of the

installation tool. Do not over-tighten.

6. 6562 Rapid Pulse DO, 6560 conductivity/ Sharp vl ah151u manual.

temperature, 6561/6579 pH, or 6565/6569

pH/ORP probes: Insert the probe into the

appropriate port, seating the pins of the two

Exo User Manual Chlorophyll Calibration Rhodamine Free

connectors. Push the probe inward so that it

seats into the port. Screw down the probe nut

by hand until it is finger tight. Use the long

end of the installation tool to tighten the probe

a quarter turn or until snug. Do not over-

tighten.

7. Ammonium, nitrate, and chloride ISE

probes do not have slip nuts and should be

installed without tools. Seat the probe body

directly on the sonde bulkhead. Use your

fingers to tighten.

!

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Calibration

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This manual is related to the following products:

600LS, 600QS, 600XLM, 600XL, 600XLM V2, 600XL V2, 600OMS V2, 6820 V2, 6920 V2, 6600EDS, 6600V2

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